A double labeling technique combining 3H-thymidine autoradiography with BrdUrd immunocytochemistry.
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منابع مشابه
Labeling of Crithidia fasciculata DNA with (3H)thymidine.
SYNOPSIS. Attempts at continuous labeling of Crithidia fasciculata D.N.A with ["H]thymidine led to a pulse-chase situation due to a ccll-mediated conversion of thymidine to thymine in the medium. The uptake of ihymine was slow compared to that of thymidine. Neither the addition of deoxyadenosine nor the sequential addition of several aliquots of ['H] thymidine had an effect on the pattern of la...
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Neurogenesis in the rat septal region was examined with 3H-thymidine autoradiography. The rats in the prenatal groups were the offspring of pregnant females given two injections of 3H-thymidine on consecutive days in an overlapping series: embryonic day (E) 13 + E14, E14 + E15,. . . E21 + E22. The rats in the postnatal groups were injected in a nonoverlapping series: the day of birth and postna...
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In order to elucidate the proliferative behavior of the intestinal metaplasia around gastric cancer, the authors used both in vitro tritiated thymidine (3H-thymidine) autoradiography and in vivo bromodeoxyuridine (BrdUrd) immunohistochemistry for labeling the proliferative cells of the normal pyloric glands and metaplastic gastric glands. The results of the methods were comparable: The labeling...
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Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incorporated into the DNA of proliferating cells. When in vivo BrdUrd infusion is coupled with bivariate flow cytometry to measure cell BrdUrd incorporation and DNA content, both the percentage of DNA-synthesizing cells [BrdUrd-labeling index (LI)] and the DNA synthesis time (TS) can be determined on the same tissue sample. From exper...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1985
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.18.267